Figure 3
From: Caffeine exacerbates seizure-induced death via postictal hypoxia
Caffeine reduced baseline brainstem oxygen. Mean partial pressure of oxygen (mmHg) pre-injection of drug or vehicle baseline (T-1); 10-min post-injection of drug or vehicle baseline (T-2); Mean 30-min post-injection of drug or vehicle baseline (T-3). (A,B) Hippocampus (HPC) and pre-Bötzinger complex (PBC) oxygen differences were not statistically significant in vehicle mice (n = 9). (C) HPC caffeine: (n = 9), T-1 = 23.51 ± 1.93, T-2 = 19.46 ± 1.72, T-3 = 15.95 ± 1.62, (ANOVA F (2, 24) = 4.567, p = 0.0209). (D) PBC caffeine: T-1 = 50.64 ± 2.72, T-2 = 36.93 ± 3.02, T-3 = 32.85 ± 1.43, (ANOVA F (2, 24) = 13.90, p =  < 0.0001***). (E) HPC CGS-21680: (n = 9), T-1 = 25.68 ± 1.80, T-2 = 27.67 ± 2.84, T-3 = 34.96 ± 2.31, (ANOVA F (2, 24) = 4.281, p = 0.026). PBS CGS-21680: T-1 = 45.03 ± 2.62, T-2 = 39.18 ± 2.98, T-3 = 56.04 ± 2.02, (ANOVA F (2,24) = 11.09, p = 0.0004***). Histobars represent mean ± SEM.
