Figure 2 | Scientific Reports

Figure 2

From: Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis

Figure 2

Enrichment and co-localization of CD147 proximal proteins within LRMs. (a) Immunoblot analysis showing the enrichment of CD147 in CSCs and NCSCs with and without MbCD treatment. The localization of CD147 to LRMs was determined after fractionation using an Optiprep™ 15–35% gradient. (b) Immunoblot analysis demonstrating the co-localization of CD147, CD133, CD44, EGFR, CD276, and MCT4 within the lipid raft fraction (20% Optiprep™) of CSCs and NCSCs. The protein levels were compared to the non-raft fraction (30% Optiprep™). (c) Immunoblot analysis of CD276, MCT4, CD147, EGFR, and MCT4 in whole cell lysates prior to lipid raft fractionation. The analysis was performed using CD147KO (CD147 single KO), CD44KO, CD133KO, CD147KO/CD44KO (double KO, CD147 double KO), and CD147KO/CD44KO/CD133KO (triple KO, CD147 triple KO) cell lines. (d) Immunoblot analysis of the same proteins in the lipid raft fraction. Full-length blots are presented in Supplementary Figs. S6, S7, S8 and S9.

Back to article page