Figure 2
ACSL1 inhibition decreases TNFα induced MMP-9 production monocytic cells. We incubated THP-1 monocytic cells with triacsin C (5 uM; ACSL inhibitor), a serine palmitoyltransferase inhibitor (myriocin, 1 μM) or a carnitine palmitoyltransferase 1 inhibitor (etomoxir, 10 μM) for 1 h and then exposed to TNFα for 24 h. We determined MMP-9 mRNA (A) and MMP-9 protein (B) using real-time PCR and ELISA, respectively. (C) The effect of TNFα stimulation on THP-1 cells in combination with inhibitors on cell viability was evaluated by measuring cell metabolic activity (MTT assay). The effect of TNFα stimulation of THP-1 cells in combination with triacsin C (5 uM), myriocin (1 μM) or etomoxir (10 μM) on cell viability. The cell viability is expressed as the percentage of cells compared to the condition of Vehicle. Three independent experiments were performed with similar results. All data are expressed as mean ± SEM (n ≥ 3). One way ANOVA (Dunnett’s Test) for comparing treatments vs control or TNFα in case of inhibitors) were used ***p < 0.001.
