Figure 1

MK3102 reduces the neurotoxicity of hemin in cultured primary cortical neurons in a concentration-dependent manner. (A) Primary cortical neurons were subjected to various concentrations of hemin and cell viability was determined by CCK-8 assay. Hemin killed primary cortical neurons. (B) The toxicity of 20 μM of hemin was attenuated by MK3102 in a concentration-dependent manner. Results are expressed as the percent of neurons remaining in culture compared with that in control cultures. Each bar is the mean ± SD of triplicate wells. (C) Representative microphotographs of MAP2 positive cells in cultured primary cortical neurons. Scale bar, 50 μm. (D) Quantitative analysis of mean fluorescence intensity of MAP2 in cultured primary cortical neurons (n = 3 per group). All data are displayed as mean ± SD. The difference between groups was analyzed using One-way ANOVA test. ***p < 0.001, ****p < 0.0001, compared with control group. #p < 0.05, ^###p < 0.0001, ^####p < 0.0001, compared with hemin + DMSO group.