Table 1 Effect of combined treatments of chlorantraniliprole (Cg) and bacterial cell suspensions [Shewanella sp. (SS4), Pseudomonas sp. (EN4) and Thauera sp. (M9)] on cumulative larval mortality of S. litura.

From: Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura (Fabricius)

Number of experiments

Treatments

Observed mortality

Expected mortality

χ2 value

Interaction

Individual treatments for experiment 1 and 2

LC50 Cg

50.0 ± 7.74a

–

–

–

LC50 SS4

46.0 ± 2.44a

–

–

–

LC50 EN4

48.0 ± 5.83a

–

–

–

LC50 M9

42.0 ± 7.34a

–

–

–

Combination treatments for experiment 1

LC50 Cg + LC50 SS4

80.0 ± 3.16b

73.0

0.67

Additive

LC50 Cg + LC50 EN4

88.0 ± 3.74b

74.0

2.64

Additive

LC50 Cg + LC50 M9

78.0 ± 2.00b

71.0

0.69

Additive

F(6, 28) – value

14.43**

–

–

–

Combination treatments for experiment 2

LC50 Cg + LC50 SS4

90.0 ± 3.16b

73.0

3.95

Synergistic

LC50 Cg + LC50 EN4

92.0 ± 3.74b

74.0

4.37

Synergistic

LC50 Cg + LC50 M9

86.0 ± 5.09b

71.0

3.16

Additive

F(6, 28) – value

18.29**

–

–

–

Individual treatments for experiment 3

LC20 Cg

24.0 ± 5.09a

–

–

–

LC50 Cg

50.0 ± 7.74ab

–

–

–

LC50 SS4

46.0 ± 2.44ab

–

–

–

LC50 EN4

48.0 ± 5.83ab

–

–

–

LC50 M9

42.0 ± 7.34a

–

–

–

Combination treatments for experiment 3

LC20 Cg + LC50 SS4

80.0 ± 6.32c

58.96

7.50

Synergistic

LC20 Cg + LC50 EN4

84.0 ± 6.78c

60.48

9.14

Synergistic

LC20 Cg + LC50 M9

74.0 ± 10.29bc

55.92

5.84

Synergistic

LC50 Cg + LC50 SS4

94.0 ± 4.0c

73.0

6.04

Synergistic

LC50 Cg + LC50 EN4

98.0 ± 2.0c

74.0

7.78

Synergistic

LC50 Cg + LC50 M9

92.0 ± 4.89c

71.0

6.21

Synergistic

F(10, 44) – value

16.88**

–

–

–

  1. Number of insecticide treatments in combination with bacterial treatments in Combined Experiment 1 = 1, Combined Experiment 2 = 3 and Combined Experiment 3 = 6. Figures are Mean ± Standard Error for observed mortality. Means followed by different superscript letters within a column are significantly different. Tukey’s test, p ≤ 0.05, **Significant at 1%64. Expected mortality ME = MB + MI (1 − MB), where MB and MI are the observed mortality percentage caused by bacteria and insecticide alone. Test for interaction based on χ2 with 1 df, using the formula χ2 = (MBI − ME)2/ME, wherein MBI is the observed mortality caused by bacteria + insecticide.