Figure 1
From: Exercise increases MEF2A abundance in rat cardiac muscle by downregulating microRNA-223-5p
Effects of exercise on GLUT4 mRNA expression and protein abundance. (a) Swimming protocol. The weight attached to the tails of the exercising rats is expressed as a percentage of body weight. Arrows indicate the timing of sacrifice. (b) Relative GLUT4 mRNA expression in rat ventricles after exercise. (c–d) Relative GLUT4 protein abundance in the membrane fraction of rat ventricles after exercise. (e–f) the corresponding values from the microsomal fraction. Representative blots of GLUT4 from two separate experiments are illustrated in the insets of panels (c–f). Letters a and c indicate control conditions, and b and d, after exercise. Actin bands were used to normalize GLUT4 density values in panels (c), (d) and (f) and Ponceau red was used in panel (e). Original blots and Ponceau images are presented in Supplementary Information Fig. 1. The bar graphs represent means ± SEMs. Each symbol represents a separate experiment. *p < 0.5, ** p < .01.
