Figure 3
From: Activation of oxidoreductases by the formation of enzyme assembly
Enzyme kinetics of (a) 100 nM POX, (b) 25 nM LOX, (c) 100 nM DLD, (d) 25 nM ALDH, and (e) 2.5 nM GDH in the absence (filled circle) and presence of 1 mM PLL (open circle). PLL, Poly-l-lysine; POX, Pyruvate oxidase; LOX, l-Lactate oxidase; DLD, d-Lactate dehydrogenase; ALDH, Aldehyde dehydrogenase; GDH, Glucose dehydrogenase. Enzyme activity measurements were carried out at pH 7 and 25 ℃ and started by the addition of the respective substrate (l-lactic acid for LOX, acetaldehyde for ALDH, d-lactate for DLD, glucose for GDH, and pyruvic acid for POX).
