Table 2 Kinetic parameters of various oxidoreductases in presence and absence of PLL.

From: Activation of oxidoreductases by the formation of enzyme assembly

Enzyme

POX

LOX

DLD

ALDH

GDH

PLL

0 mM

1 mM

0 mM

1 mM

0 mM

1 mM

0 mM

1 mM

0 mM

1 mM

KM (mM)

0.58 ± 0.06

0.82 ± 0.07

0.98 ± 0.23

0.14 ± 0.02

0.66 ± 0.15

0.93 ± 0.13

0.53 ± 0.09

0.40 ± 0.08

0.36 ± 0.03

0.31 ± 0.05

kcat (s-1)

1.81 ± 0.06

3.81 ± 0.11

2.38 ± 0.16

8.17 ± 0.16

1.1 ± 0.03

3.47 ± 0.06

3.31 ± 0.13

14.1 ± 0.06

4.54 ± 0.07

6.10 ± 0.19

kcat/KM

3.12

4.65

2.43

58.4

1.67

3.72

6.25

35.25

12.61

19.68

  1. PLL, Poly-l-lysine; POX, Pyruvate oxidase; LOX, l-Lactate oxidase; DLD, d-Lactate dehydrogenase; ALDH, Aldehyde dehydrogenase; GDH, Glucose dehydrogenase.
Back to article page