Figure 6
Efficacy in the freshly isolated pancreatic cancer tissue fragments and the generation of the diverse tumor-suppressive CMs. The single and double asterisks (comparison to CN), pound signs (comparison to CM control) and plus signs (comparison to YS) indicate p < 0.05 and 0.01, respectively. CM conditioned medium, CN control, YS YS49, PI3K activator, and CW CW008, PKA activator. (A) Diminished pancreatic cancer tissue fragments by the application of YS49-treated MSC CM in 96 h (N = 8). The red image indicates tissue fragments at 0 h, while the green image indicates tissue fragments at 96 h. (B) Generation of diverse tumor-suppressive CM from MSCs, Jurkat T lymphocytes, and PANC1 pancreatic cancer cells by activating PI3K signaling with 50 µM of YS49 and PKA signaling with 20 µM of CW008. The anti-tumor effects of varying CM were evaluated by the MTT-based viability assay with PANC1 and Pa03C pancreatic cancer cells. (C) Reduction in the level of K-Ras in PANC1 and Pa03C pancreatic cancer cells in response to the diverse tumor-suppressive CM.
