Figure 7 | Scientific Reports

Figure 7

From: Modulation of colonic immunometabolic responses during Clostridioides difficile infection ameliorates disease severity and inflammation

Figure 7

Effects of 2-DG treatment in T84 cells exposed to TcdA or TcdB. T84 cells were challenged with 100 or 250 ng/mL of TcdA or TcdB, treated with 10 mM of 2-DG and cultured for 24 h. Transepithelial electrical resistance (TEER) (A) was quantified. Expression of tight junction genes Ocludin (B, Ocln), Tight junction protein 1 (C, ZO-1), F11 receptor (D, JAM1), and proinflammatory cytokines Il1b (E), Tnfa (F) and Il8 (G) was assessed through qRT-PCR. Data expressed as mean ± SEM (n = 6–12). Asterisks mark statistical significance.*P ≤ 0.05.

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