Figure 6
From: Asarinin attenuates bleomycin-induced pulmonary fibrosis by activating PPARγ
The expression levels of P-Smad3 were reduced by asarinin, and the effect of asarinin on the expression of P-Smad3 was suppressed after PPARγ silencing. Western blotting (WB) detected the protein expression levels of P-Smad3 and Smad3 (A,B). After silencing the Pparg, WB was used to detect the protein expression levels of P-Smad3 and Smad3 (C,D). Immunofluorescence was used to detect the effect of asarinin on TGF-b-induced Smad3 nuclear translocation (magnification ×1000) (E). Data are expressed as mean ± standard deviation, and all experiments were repeated independently at least 3 times, sample size (n) = 3 for each group, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
