Figure 7
From: New drug discovery of cardiac anti-arrhythmic drugs: insights in animal models
Overview of the test protocols. Protocol I was used for whole animal experiments and Protocol II for single cell experiments. In Protocol I, after stabilization, the medium or each dose of KBR or SEA was pretreated. Then aconitine (25 g/kg, IV)bolus has been delivered). The black column indicates the duration of the arrhythmia observation. A total of 130 animals were introduced into this series of experiments. 74 were used in Protocol I, but six were excluded based on exclusion criteria. The number of animals assigned to each control (vehicle), KBR and SEA is shown in Fig. 3. In Protocol II, after stabilization, aconitine (1 M) was infused first, then vehicle, or each dose of KBR or SEA was further elaborated. A total of 13 cells were included in Protocol II(Amran MS, Hashimoto K, Homma N. Effects of sodium-calcium exchange inhibitors, KB-R7943 and SEA0400, on aconitine-induced arrhythmias in guinea pigs in vivo, in vitro, and in computer simulation studies. Journal of Pharmacology and Experimental Therapeutics. 2004 Jul 1;310(1):83–9. PMID: 15028781. https://doi.org/10.1124/jpet.104.066951) )227.
