Table 1 Biochemical characteristics of strain ICN-92133T and its phylogenomically related type strains.
Properties | 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|---|
Isolation source | Stool from a patient with Crohn’s disease | Stool from a healthy infant | Methanogen reactor | Alkaline wet woods of poplar trees | Black freshwater sediment |
Cell morphology | Rods with a rounded end | Rods with a rounded end | Curved rods | Curved rods with a rounded end | Curved rods |
Cell size (μm) | 0.4–0.5 × 1.6–3.2 | 0.4–0.6 × 1.7–3.5 | 0.3–0.5 × 1.3–2.7 | 0.6–0.9 × 3.1–7.2 | 0.3–0.6 × 2.3–4.1 |
Gram-staining | − | − (+) | − | − | − |
Catalase activity | − | − | − | − | − |
Oxidase activity | − | − | − | − | − |
Nitrate reduction activity | − | − | − | − | − |
Sporulation ability | − | − | − | − | − |
Optimal growth conditions | |||||
Temperature (20–45 °C) | 26–37 | 26–37 | 20–30 | 20–30 | 26–37 |
pH (5.0–8.5) | 6.5–8.5 | 6.5–8.5 | 6.5–7.5 | 6.0–7.0 | 6.5–8.5 |
NaCl (0–20 g/L) | < 5 g/L | < 15 g/L | < 3 g/L | < 3 g/L | < 3 g/L |
Enzymatic activity | |||||
Pyroglutamyl aminopeptidase | + | + | + | + | + |
Alkaline phosphatase | + | + | + | + | − |
Esterase (C4) | + | + | + | + | + |
Esterase lipase (C8) | + | − | + | − | − |
Leucine arylamidase | + | + | + | + | − |
Acid phosphatase | + | + | + | + | + |
Naphtol-AS-BI-phosphohydrolase | + | + | + | + | + |
α-Galactosidase | − | − | − | + | − |
β-Galactosidase | − | − | − | + | − |
α-Glucosidase | − | − | − | + | − |
β-Glucosidase | − | + | − | + | − |
N-Acetyl-β-glucosaminidase | − | − | + | + | − |
Substrate utilization | |||||
Glycerol | + | − | + | + | + |
Erythritol | − | − | + | + | NA |
d-Arabinose | − | + | − | + | NA (−) |
l-Arabinose | − | + | − | + | NA (−) |
d-Ribose | + | + | + | + | NA (−) |
Properties | 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|---|
d-Xylose | − | + | − | + | NA (−) |
d-Adonitol | + | + | + | − | NA |
d-Galactose | − | − | + | + | NA |
d-Glucose | + | + | + | + | NA (−) |
d-Fructose | + | + | + | + | NA |
d-Mannose | + | + | + | + | NA (−) |
l-Sorbose | − | − | − | + | NA (−) |
l-Rhamnose | − | − | − | + | NA |
Dulcitol | − | + | − | + | NA |
Inositol | − | + | − | + | NA |
d-Mannitol | + | + | + | + | NA (−) |
d-Sorbitol | − | − | − | + | NA |
Methyl-αd-Glucopyranoside | − | − | − | + | NA |
N-Acetyl-glucosamine | − | + | − | + | NA |
Amygdalin | − | + | − | + | NA |
Arbutin | − | + | − | + | NA |
Esculin, ferric citrate | − | + | − | + | NA |
Salicin | − | + | − | + | NA (−) |
d-Cellobiose | − | + | − | + | NA |
d-Maltose | − | − | − | + ( −) | NA (−) |
d-Lactose (bovine origin) | − | − | − | + | NA (−) |
d-Melibiose | − | − | − | + ( −) | NA (−) |
d-Saccharose (sucrose) | − | − | + | + | NA (−) |
d-Trehalose | − | − | − | + | NA (−) |
d-Raffinose | − | − | − | + | NA (−) |
Amidon (starch) | − | − | − | + | NA |
Gentiobiose | − | + | − | + | NA |
d-Turanose | − | − | − | + | NA |
d-Tagatose | − | + | + | + | NA |
l-fucose | − | − | − | + | NA |
d-Arabitol | − | + | − | + | NA |
l-Arabitol | − | + | + | − | NA |
Potassium gluconate | − | + | − | + | NA |
Potassium 5-ketogluconate | − | + | − | − | NA |
