Figure 2
From: A probabilistic view of protein stability, conformational specificity, and design
Experimental NanoLuc thermal stability comparing the wild type NanoLuc to mutants designed with ProteinMPNN (M1–M4) or BayesDesign (B1–B4). Each protein was expressed with a cell-free protein synthesis system at \(37\,^\circ\)C. Parallel aliquots were subjected to 15-min heat treatments at 37, 60, 70, 80, or \(95\,^\circ\)C and the remaining soluble protein was measured. The solubility data is the average of n = 3 biological replicates and the standard deviation error bars are shown as vertical lines. Interpolating lines show the temperature-dependent stability trend for each protein mutant.
