Figure 2
Fluorescence microscopic images of GFP-expressed E. coli incubated with PAA. (A) GFP-expressed E. coli was incubated with PAA (0, 0.0025, 0.025, and 0.25% w/v) at pH 10 or 4.0 for 3 min. Scale bar: 200 μm. The aggregates were visually observed after weak centrifugation at 2000×g for 10 s. (B) GFP-expressed E. coli was incubated with 0.25% PAA or Cy5.5-labeled PAA at pH 10 for 3 min. The fluorescent signals of GFP show the co-localization with Cy5.5-labeled PAA. Left: GFP. Middle: Cy5.5. Right: Merged image. Scale bar: 200 μm.
