Figure 5
The TnaA-Flag effect depends on its timing of appearance and proximity to the IR during larval development. (A) Developmental timeline (E, Embryo, L1-L3 larval stages) according to hours after egg laying (h AEL). The time when the expression of wg is resolved in the D/V and IR domains (green arrowheads), and the temperature shift times applied to inactivate Gal80ts to allow expression of MS1096 > tna-Flag at the dorsal hinge (pink arrowheads) are indicated. (B) TnaA and Wingless distribution in wing discs expressing tna-Flag at specific times from 36 to 90 h AEL. Note the expansion of the Wingless signal from the IR (white arrow) but not from the D/V boundary domain (empty arrowhead). (C) Expression of wg in TnaA-Flag clones in different regions of the wing disc. TnaA and Wingless immunostainings are indicated, and merged images are shown (right panels). Images from two discs show a TnaA-Flag clone at the IR (upper panel) or near the IR (middle and lower panels). A TnaA-Flag clone in the pouch does not cause an induction of wg expression (middle panel, empty arrowhead). TnaA-Flag clones at/or adjacent to the wg IR domain present the induction of wg expression (middle and lower panels, solid arrowheads). Dashed squares (middle panel) indicate the region amplified in the lower panel.
