Figure 4 | Scientific Reports

Figure 4

From: Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool

Figure 4

Luciferase activity of co-expressed plasmids in Sf21 cell-free systems. PylRS-AF was co-expressed with Adora2a-amb-Nluc. The PylRS template was directly added at the beginning of the reaction. The Adora2a-amb-Nluc template was added after the indicated time. (a) Batch based reaction showing the successful synthesis of full length protein in the presence of PylRS. The addition of the Adora2a-amb-Nluc template after 30 min resulted in the highest suppression efficiency. (b) CECF-reaction reaction showing the successful synthesis of full length protein in the presence of PylRS. The addition of the Adora2a-amb-Nluc template between 2 and 4 h resulted in the highest suppression efficiency. Measurements were performed in duplicate. Data are shown as mean ± SD.

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