Figure 3 | Scientific Reports

Figure 3

From: Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering

Figure 3

AF4 coupled SAXS analysis of LPX fractionated sample. (A) Self-assembly of mRNA LPXs by mixing of anionic mRNA (blue) with premanufactured cationic liposomes. (B) AF4-SAXS analysis of LPX formulation versus elution time with an overlay of fractograms derived from UV, LS, and SAXS including derived Rg values. The first peak, around 18 min, results from free mRNA, and the second peak, around 40 min, is from the mRNA lipoplex nanoparticles. (C) SAXS patterns derived from pure mRNA (grey) in a batch measurement compared with measurements of RNA after fractionation with AF4 (purple). (D) Comparison of a batch LPX sample with a purified LPX sample after fractionation. Note the much lower slope of the fractionated sample towards q = 0, indicative for successful separation of other particulates.

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