Figure 4 | Scientific Reports

Figure 4

From: Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering

Figure 4

Detailed analysis of the fractionated samples. Adding AF4 separation directly before the SAXS data collection allows the correlation of SAXS derived information as a function of size (elution time). (A) SAXS patterns derived from different size fractions with respective Lorentzian fits (shifted on y-axis by increasing elution time). (B) Derived parameters from peak analysis of the individual scattering curves (d-spacing in green; correlation length in blue, peak area in black). (C) Absolute material concentration (LPX conc. in mg/mL) derived from SAXS signal for LPX peak (red bars) and the calculated absolute number of particles as a function of radius of gyration (Rg) derived from MALS (green dots). (D) The total number of mRNA copies calculated from SAXS traces in certain fractions of different particle sizes (Rg from MALS) (green bars) and calculated mRNA copies per particle of size segment (red dots).

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