Figure 2
From: Selective effects of estradiol on human corneal endothelial cells
ER protein expression in human corneal endothelium. Western blotting was performed to measure protein levels of GPER (A) and ERβ (B) in native human corneal endothelium (n = 1 donor for GPER [cornea #8 in Table 1], n = 2 donors for ERβ [corneas #9 and 10]) and in the PC3 (n = 3 for each antibody) and MCF7 (n = 3 for each antibody) cell lines. Theoretical molecular weight: GPER, 42 kDa; ERβ, 55–60 kDa. The cropped blots from different gels are presented with dividing lines. Corresponding original images are provided in Supplementary Fig. 1. (C) En face immunofluorescence localization of ERβ in normal human corneal endothelium (representative images from corneas #11–13).
