Table 2 DNA fragmentation and biochemical analysis in different study groups.

	Control group (n:10)	Se group (n:10)	Low Pb group (n:10)	High Pb group (n:10)	Low Pb + Se group (n:10)	High Pb + Se group (n:10)	P value
Biochemical markers
MDA^# (nmol/mg)	0.6 ± 0.08	0.32 ± 0.05	3.73 ± 0.25^b	6.14 ± 0.36^b	1.11 ± 0.2^a,d	4.3 ± 0.4^b,f	< 0.001
TAC^# (nmol/mg)	6.7 ± 0.41	7.11 ± 0.46	2.41 ± 0.88^b	0.39 ± 0.08^b	5.45 ± 0.58^a,d	2.1 ± 0.37^b,f	< 0.001
DNA fragmentation
Tailed (%)	2.8 ± 0.84	2.8 ± 0.84	7.4 ± 1.14^b	11.6 ± 1.14^b	5 ± 1^{a c}	8.2 ± 0.84^b,f	< 0.001
Untailed (%)	97.2 ± 0.84	97.2 ± 0.84	92.6 ± 1.14^b	88.4 ± 1.14^b	95 ± 1^a,c	91.8 ± 0.84^b,f	< 0.001
Tail length (μm)	1.11 ± 0.1	1.12 ± 0.02	2.29 ± 0.14^b	3.23 ± 0.06^b	1.3 ± 0.05^a,d	2.5 ± 0.05^b,f	< 0.001
Tail DNA (%)	1.13 ± 0.08	1.14 ± .03	2.17 ± 0.11^b	3.12 ± 0.05^b	1.3 ± 0.04^a,d	2.3 ± 0.1^b,f	< 0.001
Tail moment (unit)	1.21 ± 0.2	1.31 ± 0.05	5.14 ± 0.66^b	10.09 ± 0.43^b	2.05 ± 0.34^a,d	5.67 ± 0.39^b,f	< 0.001
Photos of CCD camera of comet assay

Photos of CCD camera show effects of Pb, Se and their combinations on cerebrum comet assay in male albino rats after 4 weeks of exposure. Blue arrows = intact cells; yellow arrows = cells with a shadow, indicating damaged DNA.
^#nmol/mg, One-way ANOVA, and Tukey HSD Post-hoc Test, P > 0.05: no significant differences, P < 0.05: significant differences, P < 0.001: highly significant differences.
^aSignificant vs control group, ^bhighly significant vs control group.
^cSignificant vs low Pb group, ^dhighly significant vs low Pb group.
^eSignificant vs high Pb group, ^fhighly significant vs high Pb group.

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