Figure 4
The effect of UCMSCs on the apoptosis of chondrocytes induced by IL-β1. Chondrocytes co-cultured with UCMSCs and IL-β1 were collected to test the rate of chondrocyte apoptosis by flow cytometry. Both high (1 × 106 cells) and low (0.5 × 106 cells) doses of UCMSCs co-cultured with chondrocytes for 24 and 48 h significantly reduced the apoptosis rate of chondrocytes. Model, chondrocytes pre-treated with IL-1β; UCMSC-L, chondrocytes pre-treated with IL-1β co-cultured with a low dose of UCMSCs; UCMSC-H, chondrocytes pre-treated with IL-1β co-cultured with a high dose of UCMSCs, n = 3. *P < 0.05, **P < 0.01 analyzed by unpaired two-tailed t-test after normality and equal variance test using Shapiro–Wilk test and ANOVA plus Brown-Forsythe test in the GraphPad software.
