Figure 2
From: Intravenous immunoglobulins improve skin fibrosis in experimental models of systemic sclerosis
Histological skin evaluation and inflammatory response of all mice included in the preventive design. (a) Representative microphotographs of MGG stained skin sections scanned at 20X magnification. (b) Histograms depicting Image J software-assisted measures of dermal thickness. (c) Enlargement of a representative microphotograph where inflammatory cells are visualized as blue spots (see gray arrows). (d) Histograms of Image J software-assisted counting of cellular infiltrates in MGG stained skin sections. mRNA expression levels of pro-inflammatory cytokines, such as (e) Il1b, (f) Tnf and (g) Il6, evaluated by qRT-PCR, in frozen skin tissues. Data represent the fold change compared to PBS + 2% Maltose group and are quoted as mean ± SEM and analyzed by Kruskal–Wallis test with Dunn’s multiple comparisons; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
