Figure 1

Optimal concentrations of B. fragilis and TNF-α were determined. The CCK8 trial was designed to select the optimal concentrations of B. fragilis and TNF-α, respectively. (a) Results of B. fragilis plate counting method(1 × 109 cfu/ml). Cell viability of hcoEPIC cells co-cultured with different concentrations of (b) B. fragilis and (c) TNF-α for 24 h, respectively; ***p < 0.001, ns: not significant. All data show mean ± SEM. CCK-8, Cell Counting Kit-8.