Figure 5
B. fragilis inhibited TNF-α-induced generation of inflammation-associated cytokines. Culture supernatant was collected to value the concentrations of cytokines (a) IL-6; (b) IL-8; (c) IL-10; (d) IL-1β by Enzyme-linked immunosorbent assay. Expression of TLR2, TLR4, MYD88 were valued by western blot (e) and RT-qPCR (f–h) from molecular and protein level. ***p < 0.001, **p < 0.01, *p < 0.05 ns not significant. All data show mean ± SEM. The original western blot image was visible in Supplementary Material 2.
