Figure 6
From: Early weaning and biological sex shape long-term immune and metabolic responses in pigs
Wean age and sex effects on vaccine-induced IgG responses. Experimental design is outlined in (A) (created with BioRender.com). Blood samples were collected at 0, 7, 14, and 21 days post-vaccination with an inactivated porcine circovirus type 2 (PCV2) vaccine, and serum PCV2-IgG was quantified to determine the vaccine-specific immune response. Data are presented as means ± SE. *P < .05, #P < 0.1. N = 7–8 per experimental group, consisting of early-weaned (EW) and later-weaned (LW) female (F), intact male (IM), and castrated male (CM) pigs. Data are expressed as main effects of sex (B) and sex x EW (C). The data were subjected to a natural logarithmic transformation for normality prior to analysis but are presented as original values. A nonlinear regression revealed significant differences between EW and LW titer curves in IM (P = 0.02; Panel E) and CM (P = 0.01; Panel F), while a trend was observed in F (Panel D). Timepoint comparison statistics obtained via repeated-measures mixed-effects model with Bonferroni correction for planned comparisons.
