Figure 2
From: Neutrophil extracellular trap formation during surgical procedures: a pilot study
Microscopic analysis of NET formation. (A) Unstimulated neutrophils with intact, segmented nuclei. (B) Neutrophils stimulated with 50 nM PMA for 2 h showing loss of nuclear lobules and loss of nucleus integrity that led to an increase in overlapping staining of nuclei (histone H2B) and granule proteins (neutrophil elastase). (C) PMA-stimulated neutrophils with extensive NET formation. Photos were taken with 20X magnification, and the scale bar represents 10 µm. Fluorescent antibodies used in staining: Hoechst 33,342 for DNA (blue), rabbit anti-neutrophil elastase (green), and mouse anti-histone H2B (red).
