Figure 3
From: Neutrophil extracellular trap formation during surgical procedures: a pilot study
Neutrophil NETosis rate at different surgical time points with or without PMA induction. (A) Spontaneous NETosis rate of neutrophils without stimulation. The box and whisker plot of the dataset analysed using two-way repeated measures ANOVA, which revealed a highly significant time factor of the variance (p = 0.0043) and a significant individual difference (p = 0.018). Filled box: TAVI/PAVI group, n = 9; unfilled box: ACB group, n = 9. The centre line denotes the median value (50th percentile), while the box contains the 25th to 75th percentiles of dataset. The whiskers mark the minimum and maximum values, and all data points are marked with dots. The four different measured time points were before surgery (0 h, during induction of anaesthesia), end of surgery (closing of sternum, or end of TAVI/PAVI), 24 h and 48 h after surgery. (B) NETosis rate of neutrophils upon 50 nM PMA stimulation The box and whisker plot of the dataset was analysed using a mixed-effects model (REML). Filled box: TAVI/PAVI group, n = 9; unfilled box: ACB group, n = 9. The centre line denotes the median value (50th percentile), while the box contains the 25th to 75th percentiles of dataset. The whiskers mark the minimum and maximum values, and all data points are marked with dots. Post-hoc analysis with Turkey’s multiple comparison test correction revealed a significant drop (p = 0.0261) in neutrophil inducibility of NETosis by the end of ACB procedure.
