Figure 5

Inflammatory cytokine and chemokine analysis in the plasma. (A-K) Flow cytometric estimation of eleven inflammatory biomarkers in the plasma (EDTA) samples of both TAVI/PAVI and ACB patients. The box and whisker plots of the dataset analysed either by a mixed-effects model (REML; (A-E), (G-I), and (K)) or by two-way repeated measures ANOVA (F and J). Filled box: TAVI/PAVI group, n = 9; unfilled box: ACB group, n = 10. The centre line denotes the median value (50th percentile), while the box contains the 25th to 75th percentiles of dataset. The whiskers mark the minimum and maximum values, and all data points are marked with dots. The four different measured time points were before surgery (0 h, during induction of anaesthesia), end of surgery (closing of sternum, or end of TAVI/PAVI), 24 h and 48 h after surgery. Post-hoc analysis with Šídák’s multiple comparison test correction revealed significant regulations of IL-6, IL-8, IL-10, and MCP-1 extracellular secretion within a surgical group (*p < 0.05, **p < 0.01, ***p < 0.001).