Figure 1

SDS-PAGE, sequence analysis and phylogenetic tree of SAAP2 from MicroTom cell cultures. (A) SDS-PAGE analysis of total protein extracts before and after thermal treatment at 80 °C for 30 min obtained from tomato cell lines. Total protein extracts (30 g) were electrophoresed on 12% SDS–polyacrylamide gel and detected by Coomassie blue staining. MW: molecular weight markers. (B) Amino acid sequence deduced from the peroxidase-coding gene (LePrx21). The peptides identified by LC–MS/MS are highlighted in red. (C) Phylogenetic tree from the amino acid sequences of LePrx21 and the highly homologous peroxidases. The scale bar indicates the genetic distance among the sequences used to construct the phylogram: a distance of 0.150 means that substitutions/changes for each amino acid between two taxa have a 15% probability.