Figure 2

Electrophoretic analyses of total protein extracts obtained from cell lines of MicroTom and Moneymaker cultivars. (A) Total protein extracts (15 µg) before and after thermal treatment at 70 or 80 °C for 30 min were electrophoresed on 12% SDS–polyacrylamide gel and detected with Coomassie blue staining. MW: molecular weight markers. (B) Total protein extracts (15 µg) before and after thermal treatment at 80 °C for 30 min were electrophoresed on semi-native PAGE (12%). Following semi-native PAGE, protein bands were detected by in-gel POD activity staining using guaiacol as substrate. The results are representative of three independent experiments on three different protein extracts. Data were expressed as means ± standard deviation.