Figure 3

Gel filtration chromatography of crude extracts from MicroTom cells. Size-exclusion chromatography was performed on the YARRA™ SEC-4000 column in a 50 mM Tris–HCl buffer pH 7.5 containing 50 mM NaCl. The absorbance was measured at 280 nm. Insert: (A) SDS-PAGE (12%) and (B) semi-native PAGE (12%) analyses of MicroTom crude extracts before and after thermal treatment at 80 °C for 30 min and of PODs-fractions obtained after gel filtration chromatography. Following semi-native PAGE analysis, protein bands were detected by in-gel POD activity staining using guaiacol as substrate. MW molecular markers. The results are representative of three independent experiments on three different protein preparations. Data in Table 2 in Figure were expressed as means ± standard deviation.