Figure 6

Electrophoretic analysis of heat-treated crude extracts obtained from different plant samples. Total protein extracts (15 µg) obtained after thermal treatment at 80 °C for 30 min from cell cultures of different plants were electrophoresed. (A) semi-native PAGE (12%) analysis followed by in-gel POD activity staining using guaiacol as substrate. Lane 1: molecular weight markers; Lane 2: Daphne odora; Lane 3: Rubus idaeus; Lane 4: Psilanthus bengalensis; Lane 5: Pelargonium capitatum; Lane 6: Solanun melanogena; Lane 7: Cirsium eriophorum; Lane 8: Ficus carica grown in the light; Lane 9: Ficus carica grown in the dark. (B) SDS-PAGE (12%) analysis followed by Coomassie blue staining. The lane numbers correspond to the same samples reported in (A). The results are representative of three independent experiments on three different protein extracts.