Figure 6
From: Oxidative stress induces mitochondrial iron overload and ferroptotic cell death
Inhibition of mitochondrial iron or ROS accumulation prevented simulated ischemia/reperfusion or doxorubicin induced cell death in cardiomyocytes. (A) Mitochondrial Fe2+ levels in cardiomyocytes subjected to 6 h simulated ischemia and 12 h reperfusion (sI/R) or control condition (left panel). Mitochondrial Fe2+ levels were also measured in cells treated with 10 μM DOX or vehicle control for 12 h (right panel). *P < 0.05 vs Control or Veh. n = 4. (B) Mitochondrial lipid peroxidation assessed with MitoPeDPP in cardiomyocytes subjected to sI/R or DOX as in A. *P < 0.05 vs Control or Veh. n = 4. (C) Mitochondrial lipid peroxidation assessed with MitoPeDPP in cardiomyocytes infected with Ad-βgal, Ad-FTMT, or Ad-mCAT and subjected to sI/R or control condition. *P < 0.05 vs Control. #P < 0.05 vs Ad-βgal sI/R. n = 3. (D) Quantification of cell death from cardiomyocytes infected with Ad-βgal, Ad-FTMT, or Ad-mCAT and subjected to sI/R or control condition. *P < 0.05 vs Control. #P < 0.05 vs Ad-βgal sI/R. n = 4. (E) Western blotting for the indicated proteins from cells treated as in D. (F) Mitochondrial lipid peroxidation assessed with MitoPeDPP in cardiomyocytes infected with Ad-βgal, Ad-FTMT, or Ad-mCAT followed by treatment with DOX or vehicle control for 12 h. *P < 0.05 vs Veh. #P < 0.05 vs Ad-βgal DOX. n = 3. (G) Quantification of cell death from cardiomyocytes infected with Ad-βgal, Ad-FTMT, or Ad-mCAT followed by treatment with DOX or vehicle control for 12 h. *P < 0.05 vs Veh. #P < 0.05 vs Ad-βgal DOX. n = 4. (H) Western blotting for the indicated proteins from cells treated as in G.
