Figure 3
From: LncTUG1 promotes hepatocellular carcinoma immune evasion via upregulating PD-L1 expression
Silencing TUG1 expression reduced PD-L1 expression. (A): Relative TUG1 expression was determined by qRT-PCR. (B): The relative mRNA expression of PD-L1 was reduced in shTUG1 HCC cells. (C): The total PD-L1 protein level was reduced in shTUG1 HCC cells. (D): The PD-L1 protein level was upregulated under IFN‐γ stimulation. (E): IFN-γ stimulation restored PD-L1 protein expression in shTUG1 HCC cells. (F): Silencing TUG1 expression in HCC cells improved T cell-mediated killing in vitro. To treat HCC lines, recombinant human IFN-γ (100 ng/mL; PeproTech) was added to the medium for 24 h.
