Figure 7

Chronic consumption of HSB diet increases hippocampal glutamate levels. (A) Schematic representation of tripartite glutamatergic neurotransmission. (B) Quantification of hippocampal synaptosomal glutamate levels. (C) mRNA levels for GLUR2 AMPA subunit. (D–F) mRNA levels for NR1, NR2A, and NR2B NMDA subunit. (G) mRNA levels for mGluR5. (H) Evaluation of mRNA levels from glial glutamate transporter (GLT-1). Error bars represent the mean ± SEM; n = 4 (B), and 9–10 (C-H). Unpaired two tail T-student test (B–E,G,H), and Mann–Whitney test (F). ***p < 0.001.