Figure 4

Upregulation of GCNT1 alters oncogenic gene expression pathways in prostate cancer cells. (A) RNA-seq analysis of PC3 cells with upregulated GCNT1 identified 2445 genes that are differentially expressed in response to GCNT1. (B) Gene Ontology analysis of genes regulated by GCNT1 shows an enrichment of genes with roles in ‘signalling’ and ‘cell communication’. (C) Ensemble Gene Set Enrichment Analysis of genes regulated by GCNT1 reveals downregulation in the ‘epithelial mesenchymal transition’ hallmark signature, and downregulation in ‘P53’ and ‘MEK’ oncogenic signatures. (D) Heatmap to illustrate 53 genes with roles in EMT that are significantly downregulated in GCNT1 overexpressing cells. (E) Validation at the protein level shows Galectin-1, which is encoded by the LGALS1 gene, is downregulated when GCNT1 is overexpressed. (F) Analysis of the TCGA PRAD cohort (n = 493)^31,35,36 shows a significant correlation between GCNT1 and LGALS1 mRNA levels in primary prostate cancer clinical tissue.