Figure 4
From: Construction of IgG–Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction
(a) Fluorescent microscopy image of SK-BR-3 cells. Top panels: fluorescent images. Bottom panel: bright field images of the same observation areas of the fluorescent images. The pictures indicate 100-μm scale bars. Antibody combinations for fluorescence imaging is illustrated on the right. (b) Flowcytometry analyses using Her2 positive (SK-BR-3) or CD3 positive (HPB-ALL) cells. Black: negative control (w/o antibody). Blue: positive control using anti-Her2 or anti-CD3 antibodies. Red: IgG–Fab2. (c) In vitro cytotoxicity assay. Target cell: Her2+ SK-BR-3 cell. Effector cell: LAK T-cell. E/T ratio = 5. Standard deviations of the determined cytotoxicity values are indicated with error bars (n = 4).
