Figure 4
A typical chromatography profile of ammonium sulfate fraction of Sarcocystis spp. protease from infected water buffaloes on DEAE-Sepharose (15 × 1.6 cm) pre-equilibrated with 50 mM Tris–HCl buffer, pH 7.0. Three ml fractions were collected at a flow rate of 60 ml/h and 4 °C.
