Figure 2 | Scientific Reports

Figure 2

From: CSE1L is a negative regulator of the RB-DREAM pathway in p53 wild-type NSCLC and can be targeted using an HDAC1/2 inhibitor

Figure 2

Depletion of CSE1L induces a p53/p21 dependent downregulation of AURKA pathway genes and activation of RB. A549 p53+/+ and p53−/− cells were transfected with control siRNA or CSE1L RNA for 48 h. mRNA was qPCR analyzed for the indicated genes. Average (from three experiments) relative mRNA is presented with SD indicated. Statistical differences are indicated (* p < 0.05, ** p < 0.01, *** p < 0.001) (A). Lysates were immunoblotted for the indicated proteins (representative images of three independent experiments) (C). (B) A549 p53+/+ cells were transfected with control siRNA, CSE1L RNA, and/or p21 siRNA for 48 h. mRNA was qPCR analyzed for the indicated genes. Average (from three experiments) relative mRNA is presented with SD indicated. Statistical differences are indicated.

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