Figure 3 | Scientific Reports

Figure 3

From: CSE1L is a negative regulator of the RB-DREAM pathway in p53 wild-type NSCLC and can be targeted using an HDAC1/2 inhibitor

Figure 3

Depletion of CSE1L induces a more pronounced cytotoxicity in p53 WT NSCLC cells. A549 p53+/+ and p53−/− cells were transfected with control siRNA or CSE1L siRNA for 72 h and then analyzed with MTT assay (A) or FACS analysis for sub-G1 (B). Average (8 replicates) relative MTT absorbance and average (triplicate) % sub-G1 cells are presented with SD indicated. (C) A549 p53+/+ and p53−/− cells were transfected with control siRNA or CSE1L siRNA for 48 h and then plated for colony formation assay. Average (triplicate) % colony formation is presented with SD indicated. Representative images are presented. (D). A549 p53+/+ and p53−/− cells were treated with vehicle or onvansertib (1 µM) for 72 h and then analyzed for sub-G1 cells. % sub-G1 cells are presented with SD indicated. Statistical differences (* p < 0.05) are indicated in (A), (B), and (C). There is no significant difference in D.

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