Figure 5 | Scientific Reports

Figure 5

From: Repurposing disulfiram, an alcohol-abuse drug, in neuroblastoma causes KAT2A downregulation and in vivo activity with a water/oil emulsion

Figure 5

Disulfiram release from the emulsion in vitro and in vivo. (A) The disulfiram release profile from the developed 62:38 emulsion at room temperature (mean ± standard error of the mean, n = 3). (B) MYCN-amplified N91 neuroblastoma cells were treated with 62:38 emulsions containing 25, 50, 250 and 500 nM disulfiram for 48 h. Representative western blots for MYCN and actin in untreated N91 cells (Ctl) and those exposed to empty (Veh.) or disulfiram-loaded emulsions (n = 2) and quantified MYCN levels relative to actin are shown. (C) Disulfiram absorption in the intraperitoneal liquid of treated mice (n = 8) was measured by HPLC–UV.

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