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Figure 2

From: The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella

Figure 2

Prevotella have the enzyme Rnf. Rnf is evident in the (A) genome, (B) proteome, and (C) measurements of enzyme activity. Figures shown on the left side are for Prevotella brevis GA33, while figures shown on the right side are for Prevotella ruminicola 23. Results in (C) are mean ± standard error of 3 biological replicates (cell membranes prepared from independent cultures). Abbreviations: Fdox, oxidized ferredoxin; Fdred, reduced ferredoxin (two reduced iron-sulfur clusters); NADox, oxidized NAD; NADred, reduced NAD; DDA, data-dependent acquisition; DIA, data-independent acquisition; Membrane, cell membrane sample; Solb membr, solubilized cell membrane sample. See Supplementary Tables S3 and S4 for more information.

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