Figure 5 | Scientific Reports

Figure 5

From: Validation of airway porcine epithelial cells as an alternative to human in vitro preclinical studies

Figure 5

Human and porcine airway epithelial cells in submerged differentiative conditions. IF analysis of human and porcine tracheal and bronchial epithelial cells cultured for 30 days in B-ALI™ differentiation medium. (a,b) Basal cytokeratin 5 and cytokeratin 14 (green) were expressed at different levels in the submerged stratified cultures. (c) All human and porcine cells were CK7 positive (red), while the tightness of the stratified epithelium was highlighted by ZO-1 protein (green). (d) Only a few proliferative Ki67 positive cells were detected in all the analysed epithelial cell cultures. (e,f) The two airway-specific markers MUC5AC and acetylated α-tubulin were expressed at high levels after 30 days in differentiative culture conditions. Nuclei were counterstained with DAPI, scale bar = 50 µm.

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