Figure 3
From: Gliotoxin-mediated bacterial growth inhibition is caused by specific metal ion depletion
Factors influencing E. faecalis growth and phenotype related to Zn2+ availability. (A) Relief of TPEN-mediated growth inhibition of E. faecalis by Zn2+ addition (0—200 µM). (B) GT (up to 10 µg/ml) augments vancomycin-mediated growth inhibition of E. faecalis in a concentration-dependent manner. E. faecalis OD600 0.2 = 1.2 × 108 CFU/ml. (C) Zn2+ (200 µM) relieves GT augmentation of vancomycin-mediated growth inhibition of E. faecalis. (D) A speckled growth phenotype of E. faecalis unique to the presence of vancomycin (4 µg/ml) is decreased in the presence of gliotoxin. Zn2+ addition (200 µM) reverses the GT dissipation effect and restores the speckled growth phenotype.
