Figure 3

Eukaryotic microbial diversity and taxonomic abundance based on 18S amplicon sequencing data. (A) The observed species, Chao1, Shannon, Simpson, InvSimpson, and Fisher diversity (Alpha diversity) measures were used to estimate the Eukaryotic microbial community diversity of Cox's Bazar and Saint Martin samples as described for the prokaryotic microbes. (B–G) Beta diversity of the eukaryotic microbial community was estimated here as described in Fig. 2B–G. For the two sample sites, Bray, Weighted-Unifrac, and Unweighted-Unifrac distance measures were used. Permutational multivariate analysis of variance (PERMANOVA) was performed with 999 permutations to estimate a significance (p value) for differences between two locations. PERMANOVA with 999 permutations was used to determine the significance (p value) of differences between two locations. Significance level (p value) 0.0001, 0.001, 0.01, 0.05, and 0.1 are represented by the symbols "****", "***", "**", "*", and "n.s", respectively. Stress value represents the goodness of fit of NMDS (> 0.2 Poor, 0.1–0.2, Fair, 0.05–0.1 Good, and < 0.05 Excellent). (H) Comparison of relative abundance of twenty-five eukaryotic divisions and (I) Order in the two different locations (Cox’s Bazar and Saint Martin). The diversity for each order is plotted and differences were tested using Wilcoxon sum rank test. Significance level (p value) 0.0001, 0.001, 0.01, 0.05, and 0.1 are represented by the symbols “****”, “***”, “**”, “*”, and “n.s”, respectively.