Figure 3 | Scientific Reports

Figure 3

From: Patient-specific primary and pluripotent stem cell-derived stromal cells recapitulate key aspects of arrhythmogenic cardiomyopathy

Figure 3

ACM cardiac stromal cells show fibro-adipogenic differentiation. (A) Representative images of Nile Red staining of hiPSC-D and Primary cells from HC subjects and ACM patients cultured in adipogenic medium for 3 days. Nuclei are stained with Hoechst 33,342. Magnification is 40X and the scale bar indicates 50 μm. (B) Quantification of Nile Red has been shown as fold change compared to HC hiPSC-D in the graphs (n = 3 HC hiPSC-D vs. ACM hiPSC-D vs. HC Primary vs. ACM Primary; Two-way ANOVA and Tukey’s post-test). (C) Representative images of immunostaining for COL1A1 of hiPSC-D and Primary cells (HC and ACM) cultured in low serum (2%) growth medium and stimulated with TGF-β1 for 3 days. Nuclei are stained with Hoechst 33,342. Magnification is 40X and the scale bar indicates 50 μm. (D) Quantification of COL1A1 has been shown as fold change compared to HC hiPSC-D in the graphs (n = 3 HC hiPSC-D vs. ACM hiPSC-D vs. HC Primary vs. ACM Primary; Two-way ANOVA and Tukey’s post-test). Data information: mean. *P < 0.05 and **P < 0.01.

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