Figure 1 | Scientific Reports

Figure 1

From: A fixed 20:1 combination of cafedrine/theodrenaline increases cytosolic Ca2+ concentration in human tracheal epithelial cells via ryanodine receptor-mediated Ca2+ release

Figure 1

Intracellular Ca2+ measurements were performed on Human tracheal epithelial cells (HTEpC). Intracellular Ca2+ concentrations ([Ca2+]i) were recorded as Fura-2/AM (Fura-2) fluorescence intensity ratio of 340:380 nm excitation. (A) When FURA-2 340/380 ratio was normalized after a 100-s resting period, fluorescence ratio remained constant under resting conditions during the 800-s observation period, demonstrating no changes in [Ca2]i. Arrows indicate different timepoints of the FURA-2 340/380 ratio, which are illustrated in (B1–B3) after conversion into false colors. Potassium chloride (K+, 200 mM) completely inverted the membrane potential, leading to a strong increase in FURA-2 340/380 fluorescence of human tracheal epithelial cells.

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