Table 1 List of murine ryanodine receptor (RyR) primers used for RT-PCR and their specific annealing temperatures.
Target | Gene | Sequence | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
RyR 1 | Ryr1 | Forward: 5’-CGCTCCCACTTCATCCCTAC-3’ | 385 | 55 |
Reverse: 5’-CTCCTGCCTTGGCCATTTTG-3’ | ||||
RyR 2 | Ryr2 | Forward: 5’-ACCTACTCCGAAGGCTGGTGTT-3’ | 148 | 55 |
Reverse: 5’-TTCTTCCGAGGCAGCACCAAAG-3’ | ||||
RyR 3 | Ryr3 | Forward: 5’-GACAGGACCAGGAACGGAAG-3’ | 315 | 58 |
Reverse: 5’-GCTCCACCGTCTTTTCTGGA-3’ |
