Figure 1

Initial clustering of BALF cells (a) Graphical illustration of the study design. The figure was created using the BioRender software. (b) UMAP representation of 63,022 equine BALF cells colored by the six major cell groups: alveolar macrophages (28,277 cells), proliferating macrophages (3420 cells), T cells (28,112 cells), mast cells (1257 cells), neutrophils (797 cells) and dendritic cells (1339 cells). (c) Example of expression of specific markers for the different cell types. (d) Heat map demonstrating the difference in expression levels of the top biomarker genes for each of the six major clusters. (e) Proportion of cell-types in the EA and healthy control horses, by group.